Phytochemical Screening of Leaf and Root Extracts of Carica papaya (Pawpaw) from Asaba, Delta State, Nigeria
Keywords:
Phytochemicals, Carica papaya leaves, roots, plant extract, Cardiac glycosidesAbstract
A rekindled interest in the Pharmaceutical importance of plants has led to the discovery and adoption of plant extracts, which were commonly used in medicine as an alternative source of remedies. This study evaluated the phytochemical constituents in Carica papaya leaf and root extract using standard methods. The aqueous extract of the leaves analyzed revealed the presence of tannins (0.002±0.001), flavonoids (0.020±0.002), phenolics (0.011±0.002), and alkaloids (0.024±0.003), while that of the roots gave tannins (0.100±0.010), flavonoids (0.019±0.001), phenolics (0.016±0.002), and alkaloids (0.027±0.001). Cardiac glycosides, phlobatannins, and triterpenes were not detected in the leaves and root extract of the plant. The high alkaloid content means the leaves and the roots are medicinally efficacious. The result obtained in this study showed the scientific basis for the effectiveness of this plant in the treatment of various ailments and diseases, as well as its uses in industries and medical sciences.
References
Abhishek Mathur, Stish K, Verma, Santosh, K, Singh, G., & Prasad, V. K. (2011). Investigation of the antimicrobial, antioxidant, and anti-inflammatory activity of a compound isolated from Murraya koenigii. International Journal of Agriculture and Biological Technology, 2(1), 270-282.
Adachukwu, P. I., Ann, O., & Faith, E. U. (2013). Phytochemical analysis of pawpaw (Carica papaya) leaves. International Journal of Life Sciences and Biotechnology, 1(1), 1-5.
Adetuyi, A. O., & Popoola, A. V. (2010). Extraction and dyeing potential studies of the colorant in Zanthoxylum zanthoxyloides plant on cotton fabric. Journal of Science Engineering Technology, 8(2), 329-3299.
Agbonghae, O. A., & Nwokoro, S. O. (2019). Yield, proximate and mineral composition of pawpaw (Carica papaya L.) leaf protein concentrate, leaf meal, and bagasse. International Journal of Life Science Research, 7(2), 246-251.
Akash, J., Subhiksha, S., Ramesh, V., Abishek, M., Jaishree, M., Rishi Tushaar, V., Harish Kumar, S., Siva Kumar, B., & Ilango, K. (2023). Carica papaya’s anti-diabetic and anti-cancer properties: A review. Journal of Advanced Zoology, 44(3), 322-333.
Alara, N. R., Abdurahman, N. H., & Alara, O. R. (2022). Carica papaya: A comprehensive overview of the nutritional values, phytochemicals, and pharmacological activities. Advances in Traditional Medicine, 22, 17-47. Aravind. G, Debjit Bhowmik, Duraivel. S., & Harish. G. (2013). Traditional and medicinal uses of Carica papaya. Journal of Medicinal Plant Studies, 1(4), 2320-3862.
Aravind, S. M., Wichienchot, S., Tsao, R., Ramakrishnan, S., & Chakkaravarthi, S. (2021). Role of dietary polyphenols on gut microbiota, their metabolites, and health benefits. Food Research International, 14(2), 110189.
Ayoola, P. B., & Adeyeye, A. (2010). Phytochemical and nutrient evaluation of Carica papaya (pawpaw) leaves. International Journal of Research and Reviews in Applied Sciences, 5(3), 325-328.
Biswas, B., Kimberly, F. M., Dwayne, D., & Anand, Y. (2013). Antimicrobial activity of Guava leaf extracts (Psidium guajava) on two Gram-positive and two Gram-negative bacteria. International Journal of Microbiology, 2, 7-15.
Chávez-Pesqueira, M., & Núñez-Farfán, J. (2017). Domestication and genetics of papaya: A review. Frontiers in Ecology and Evolution, 5, 155.
Ciulci, I. (1994). Methodology of analysis of vegetable drugs. Chemical Industries Branch, Division of Industrial Operations. UNIDO, p. 24-67.
Cushnie, T. P. T., & Lamb, A. J. (2011). Recent advances in understanding the antibacterial properties of flavonoids. International Journal of Antimicrobial Agents, 38(2), 99-107.
Dwivedi, M. K., Sonter, S., Mishra, S., Patel, K., & Singh, P. K. (2020). Antioxidant, antibacterial activity, and phytochemical characterization. AOAC. Official methods of analysis of AOAC International, 17th Edition. AOAC.
Elansary, H. O., Szopa, A., Kubica, P., Ekiert, H., & Ali, H. M. (2018). Bioactivities of traditional medicinal plants in Alexandria. Evidence-Based Complementary and Alternative Medicine, 32(1), 1-13. Isaac, A. O., & Adekoya, A. (2020). Phytochemical and Antimicrobial Screening of Root Extracts of Carica papaya. Nigerian Journal of Pharmaceutical and Applied Science Research, 5(2), 18-24.
Irabor, E. E. I., Eze, P. N., Unuigbe, C. A., Imafidon, M. I., Omoruyi, U., & Emmanuel, V. (2023). Proximate analysis and mineral determination of Calliandra surinamensis seed. Journal of Chemical Society of Nigeria, 48(1), 183-191. Krishna, K. L., Paridhavi, M., & Patel, J. A. (2008). Review on nutritional, medicinal and pharmacological properties of papaya (Carica papaya Linn.). Nat Prod Radiance, 7(4), 364–73.
Martel, J., Ojcius, D. M,, Ko, Y. F., & Young, J. D. (2020). Phytochemicals as prebiotics and biological stress inducers. Trends Biochemical Sciences, 45, 462-471.
Mehta, Kavit., Patel, B. N. & Jain, B. K (2013). Phytochemical analysis of the leaf extract of Phyllanthus fraternus. Research Journal of Recent Science, 2(12), 12-15.
Nduche, M., Nkaa, F., & Onyebinime, A. (2019). Phytochemical Screening and Antimicrobial Activity of Carica papaya, Citrus paradis, Citrus sinesis and Vernonia amyadalina, Journal of Diary and Vetenary Sciences, 9(4), 555768 doi;10.1908/JDVS.2019.09555768
Plamada, D., & Vodnar D. C. (2022). Polyphenols gut microbiota interrelationship: A transition to a new generation of prebiotics. Nutrients. 14(1), 137-143. Rahmani, A. H., & Aldebasi, Y. H. (2016). The potential role of Carica papaya and their active constituents in preventing and treating diseases. International Journal of Pharmacology and Pharmaceutical Sciences. 8(1), 11–15.
Rivera-Pastrana, D. M., Yahia, E. M., & Gonzales-Aguilar, G. A. (2010). Phenolic and carotenoid profiles of papaya fruit (Carica papaya L) and their contents under low temperature storage. Journal of Scientific Food Agriculture, 90(14), 2358-2365.
Sarkar, D., Shetty, A., & Fauzdar, A. (2020). Cytotoxic and apoptotic effects of Carica papaya on breast cancer cell line. Indian Journal of Pharmaceutical Education and Research, 54(2), 273-278.
Singh, S. P., Kumar, S., Mathan, S. V., Tomar, M. S., Singh, R. K., Verma, P. K., Kumar, A., Singh, R. P., & Acharya, A. (2020). Therapeutic application of Carica papaya leaf extract in the management of human diseases. Daru, 28(2), 735-744.
Sofowora, A. (1993). Medicinal plants and traditional medicines in Africa. Spectrum Books Ltd, pp 191-289
Subrata, K., Kumar, P., Suman, K. S., Bhaskarivoti, G., Gogoi, E.-R., & Hahm, H. (2023). Phytochemicals in cancer prevention and therapeutics: Current advancement and future perspective. Frontiers in Pharmacology, 14, 1156197.
Titanji, V. the P., Zofou, D., & Ngemenya, M. N. (2008). The anti-malaria potential of medicinal plants used to treat Malaria in Cameroonian Folk Medicine. African Journal of Traditional, Complementary and Alternative Medicines, 5(3), 302-321.
Trease, G. E., & Evans, W. C. (1989). Pharmacognosy (13th Edition). Baillere Tindall, pp 176-180.
Ukpo, G. E., Owolabi, M. A., Imaga, N. O., Oribayo, O. O., & Ejiroghene, A. J. (2017). Effect of carica papaya (Linn) aqueous leaf extract on the pharmacokinetic profile of ciprofloxacin in rabbits. Tropical Journal of Pharmaceutical Research, 16(1), 127-134.
UN Food and Agriculture Organization Statistical Database (FAOSTAT). (2022). Papaya production in 2020. FAO.
Wadood, A., Gufran, M., Jamal, B. S., Naeem, M., Khan, A., Ghaffar, R., & Asnad (2013). Phytochemical analysis of medicinal plants occurring in the local area of Mardan. Biochemistry and Analytical Biochemistry, 2(14), 1-44.
Yahaya, A., Ali, M., & Idris, A. (2017). Antibacterial activity and phytochemical screening of carica papaya on some enteric bacterial isolates of public health importance. Greener Journal of Biological Sciences, 7(1), 1-7.
