Comparative Amino Acid Profiling of Arthropod Haemolymph in Zaria, Nigeria
Keywords:
Arthropod, Arthropod Haemolymph, Amino Acid, Kaduna State, NigeriaAbstract
This study investigates the amino acid profile of Haemolymph in eight arthropod species from Zaria, Nigeria, representing the classes Insecta, Arachnida, and Crustacea. Haemolymph samples (1 mL) were collected using the novel Antennae method from anesthetized and cleaned arthropods. Amino acid analysis was conducted using the Applied Biosystems PTH Amino Acid Analyzer following hydrolysis and defatting. A total of 17 amino acids were detected in the Haemolymph, including leucine, lysine, isoleucine, phenylalanine, serine, aspartic acid, valine, methionine, proline, arginine, tyrosine, histidine, cystine, alanine, glutamic acid, glycine, and threonine. Notably, Glutamic acid was found to be the most abundant in spider (5.08g/100g) while cystine was the least abundant in cricket (0.02 g/100g). Statistical analysis revealed significant differences (P<0.05) in amino acid composition between arthropod species and compared to RPMI 1640 control. These results suggest that amino acids play a critical role in cellular processes, including protein synthesis, energy production, and immunity, aiding in the arthropods' defense against infections. This finding aligns with previous studies, highlighting the importance of these compounds in arthropod physiology and their potential for further studies on metabolic processes and immune responses in invertebrates.
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