Assessment of antimicrobial activity of Landolphia owariensis and Nuclea latifolia plant used as anti-diarrhoea in Walijo
Keywords:
Antidiarrheal, Landolphia owariensis, uclia latifolia, Infectious Disease, Antidiarrhoeal ScreeningAbstract
Despite the success of antibiotic discovery, infectious diseases are consistently ranked second among causes of death worldwide. The antidiarrhoeal activities of the crude extracts from the leaves Landolphia owariensis and Nuclea latifolia were carried out using standard methods to assess new active compounds with therapeutic potential for infectious diseases. The results of the antidiarrhoeal screening of the crude extracts showed that Methanol, petroleum ether and ethyl acetate extracts of L. owariensis gave 9.3 g, 8.2 g, and 6.3 respectively, while extracts of N. latifolia gave 8.4 g, 5.5 g, and 4.5 g respectively. The extracts were tested against selected enteric pathogens of different concentration. Nuclea latifolia in ethyl-acetate and methanol was highly effective against S. typhi with a resistance observed when tested against S. dysentriea at some conditions and E. coli respectively. The minimum inhibitory concentration was 250 μg/cm3 for S. typhi. Petroleum ether showed moderate effectiveness against Salmonella typhi and highly effective against S. dysentriea. While for E. coli, a complete resistance with minimum inhibitory concentration (MIC) is 500 μg/cm3 for S. typhi and 1 mg/cm3 for E. coli. The degree of effectiveness is observed to be increasing with increased in the concentrations of solvent extract. However, N. latifolia extract has shown to be more effective on S. typhi followed by shigella dysentriea. The minimum inhibitory concentration (MIC) for Salmonella typhi and Shigella dysentriea is 2 mg/cm3 respectively. But the wide range of inhibitory properties shown by L. owariensis extract against the tested organisms indicates its potential against the diarrhoea causing microbes.
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