SPECTROSCOPIC CHARACTERIZATION AND IDENTIFICATION OF FRIEDELAN-3-OL FROM MANGIFERA INDICA ROOT AND ITS ANTI-MICROBIOLOGICAL ACTIVITIES AGAINST SOME CLINICALPATHOGENS

Abstract

Plants used in ethnomedicine have remained a foundation of therapeutic agents in nature till date. Recently, there has been wider interest in research for new agents for treatment of both emerging and re-emerging infectious diseases as a result of anti-microbial resistance to drugs. The application of some common drugs which results in the development of their side effects, resistance and other deficiencies created the need for use of alternative natural remedies with better therapeutic properties. This research sought to contribute to this quest by extracting and identifying possible therapeutic compounds from the roots of Mangifera indica, as well as
evaluating their efficacy against a variety of clinical microbiological infections. Using a Soxhlet device, the powdered root was extracted with ethyl acetate and methanol. The extracts were separated on a silica gel column and eluted in ethyl acetate with increasing quantities of methanol. Fractions obtained were examined by some spectroscopic methods such as TLC, UV, IR and based on their profiles 40 fractions obtained were pooled to four combined fractions which were analysed using NMR (1D and 2D) to confirm the structures of their constituents. The result showed that MI-03 is a triterpenoid (Friedelan-3-ol). The antimicrobial results showed
high sensitivity of Vancomycin resist Enterococci (30mm), Staphylococcus aureus (28mm), Escherichia coli (31mm) and Pseudomona aeruginosa (30mm) to the isolated triterpenoid (Friedelan-3-ol), but Methicillin resist Staphylococcus aureus and Salmonella typhi was resistant to the compound. The fungi Aspergillus fumigates (30mm), Aspergillus nigre (31mm), Fibrophoria vaillentii (29mm), Fomitopsis pinicola (31mm), Rhizopus SP (20mm) and Serpula lacrymans (30mm) were sensitive to the isolated compound, while Aspergillus flavus, Coniophora puteana, Fusarium oxysporum, Fusarium proliforatum and Sclerotium rofsii were resistant to the
compound. The sensitivity of these clinical pathogens indicates that the isolated natural organic triterpenoid (Friedelan-3-ol) canbe harnessed, prepared and used for their treatment.